Assay of serum fructosamine: internal vs external standardization.
نویسندگان
چکیده
2. Galen RS. The enzyme diagnosis of myocardial infarction. Hum Pathol1975;6:14155. 3. Lott JA. Creatine kinase, lactate dehydrogenase and their isoenzymes in clinical medicine. Corning, NY: Corning Medical, Corning Glass Works, 1984. 4. Yusuf S, Collins R, Lin L, Sterry H, Pearson M, Sleight P. Significance of elevated MB isoenzyme with normal creatine kinase in acute myocardial infarction. Am J Cardiol 1987;59:245-9.
منابع مشابه
Assay of serum fructosamine that minimizes standardization and matrix problems: use to assess components of biological variation.
Methodological problems with the assay of fructosamine in serum--standardization, matrix effects, and dependence on buffer pH--have been minimized with a method involving colorimetric assay of each specimen and subsequent re-assay after standard addition of 1-deoxy-1-morpholinofructose. Absorbance at optimum wavelength of 540 nm varies linearly with fructosamine concentration to at least 5.5 mm...
متن کاملStandardization of serum fructosamine assays.
We have calibrated a secondary serum protein standard by use (as primary standards) of samples of albumin and polylysine glycated with [14C]glucose in vitro, the glycation of which was assessed by radioactivity measurements and by elementary analysis for C and N. Using this standard for calibration in our improved fructosamine assay, one obtains an average fructosamine value of 247 mumol/L for ...
متن کاملRelative potency of commercial calibrators for fructosamine, and their effect on measurements of fructosamine in serum.
The protein-based secondary standardsfor serum fructosamine in a commercial test kit (Roche Diagnostics) have recently been modified by the manufacturer. Under certain assay conditions, the new calibrator gave significantly greater serum fructosamine values than those obtained with the originally supplied calibrator. However, for 20-4, samples and absorbance measurements at 10 and 15 mm, hereaf...
متن کاملAutomated fructosamine assay with improved accuracy used to quantify nonenzymatic glycation of serum proteins in diabetes mellitus and chronic renal failure.
We optimally solubilized the diformazan that forms during the fructosamine reaction with a nonionic detergent; we then automated the assay. After optimal solubilization of diformazan, we found that serum urate contributed significantly to the color formation in fructosamine reaction. When the error introduced by urate was corrected mathematically by use of an experimental determined factor, the...
متن کاملIs serum fructosamine assay specific for determination of glycated serum protein?
We compared the fructosamine activity in sera from healthy and diabetic subjects with the degree of protein glycation detected by a liquid-chromatographic method. The latter technique measures furosine as a specific product after hydrolysis of epsilon-amino-fructose-lysine. Our results indicate that the fructosamine assay measures the extent of glycation of purified human serum albumin correctl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 33 10 شماره
صفحات -
تاریخ انتشار 1987